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All patients had 50 mL of whole blood taken using a special syringe with increased inner surface area (Orthogen, Düsseldorf, Germany).Medical-grade glass beads in the special syringes increase the nonpyrogenic surface area. These glass spheres induce the dose-dependent production of IL-1Ra by white blood cells in whole blood incubated at 37°C. After incubation, the blood-filled syringes were centrifuged, and the serum supernatant was filtered (0.22 μm; Millipore, Carrigtwohill, Co. Cork, Ireland) and aliquoted into 6–8 2 mL portions.

It’s given as a day care injection

Patient can walk pain free after that.

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August 2023

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